LOCUS       sequence-84520-e        5196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  full-length native Gal4 with stop in Gateway middle entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL
  TITLE     Gal80 intersectional regulation of cell-type specific expression in 
            vertebrates.
  JOURNAL   Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 
            2011 Sep 8.
  PUBMED    21905164
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.22734"; journalName: "Dev Dyn"; date: "2011-10"; 
            volume: "240"; issue: "10"; pages: "2324-34"
COMMENT     Sequence Label: pME-Gal4
FEATURES             Location/Qualifiers
     source          1..5196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             674..3319
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    complement(3321..3420)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3437..3456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3438..3456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3461..3477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3461..3477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3579..3598)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3590..4399
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3657..3676)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4546..5134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5035..5054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctag accatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      721 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      781 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      841 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      901 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      961 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
     1021 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
     1081 tagtaacaaa ggtcaaagac agttgactgt atcgattgac tcggcagctc atcatgataa
     1141 ctccacaatt ccgttggatt ttatgcccag ggatgctctt catggatttg attggtctga
     1201 agaggatgac atgtcggatg gcttgccctt cctgaaaacg gaccccaaca ataatgggtt
     1261 ctttggcgac ggttctctct tatgtattct tcgatctatt ggctttaaac cggaaaatta
     1321 cacgaactct aacgttaaca ggctcccgac catgattacg gatagataca cgttggcttc
     1381 tagatccaca acatcccgtt tacttcaaag ttatctcaat aattttcacc cctactgccc
     1441 tatcgtgcac tcaccgacgc taatgatgtt gtataataac cagattgaaa tcgcgtcgaa
     1501 ggatcaatgg caaatccttt ttaactgcat attagccatt ggagcctggt gtatagaggg
     1561 ggaatctact gatatagatg ttttttacta tcaaaatgct aaatctcatt tgacgagcaa
     1621 ggtcttcgag tcaggttcca taattttggt gacagcccta catcttctgt cgcgatatac
     1681 acagtggagg cagaaaacaa atactagcta taattttcac agcttttcca taagaatggc
     1741 catatcattg ggcttgaata gggacctccc ctcgtccttc agtgatagca gcattctgga
     1801 acaaagacgc cgaatttggt ggtctgtcta ctcttgggag atccaattgt ccctgcttta
     1861 tggtcgatcc atccagcttt ctcagaatac aatctccttc ccttcttctg tcgacgatgt
     1921 gcagcgtacc acaacaggtc ccaccatata tcatggcatc attgaaacag caaggctctt
     1981 acaagttttc acaaaaatct atgaactaga caaaacagta actgcagaaa aaagtcctat
     2041 atgtgcaaaa aaatgcttga tgatttgtaa tgagattgag gaggtttcga gacaggcacc
     2101 aaagttttta caaatggata tttccaccac cgctctaacc aatttgttga aggaacaccc
     2161 ttggctatcc tttacaagat tcgaactgaa gtggaaacag ttgtctctta tcatttatgt
     2221 attaagagat tttttcacta attttaccca gaaaaagtca caactagaac aggatcaaaa
     2281 tgatcatcaa agttatgaag ttaaacgatg ctccatcatg ttaagcgatg cagcacaaag
     2341 aactgttatg tctgtaagta gctatatgga caatcataat gtcaccccat attttgcctg
     2401 gaattgttct tattacttgt tcaatgcagt cctagtaccc ataaagactc tactctcaaa
     2461 ctcaaaatcg aatgctgaga ataacgagac cgcacaatta ttacaacaaa ttaacactgt
     2521 tctgatgcta ttaaaaaaac tggccacttt taaaatccag acttgtgaaa aatacattca
     2581 agtactggaa gaggtatgtg cgccgtttct gttatcacag tgtgcaatcc cattaccgca
     2641 tatcagttat aacaatagta atggtagcgc cattaaaaat attgtcggtt ctgcaactat
     2701 cgcccaatac cctactcttc cggaggaaaa tgtcaacaat atcagtgtta aatatgtttc
     2761 tcctggctca gtagggcctt cacctgtgcc attgaaatca ggagcaagtt tcagtgatct
     2821 agtcaagctg ttatctaacc gtccaccctc tcgtaactct ccagtgacaa taccaagaag
     2881 cacaccttcg catcgctcag tcacgccttt tctagggcaa cagcaacagc tgcaatcatt
     2941 agtgccactg accccgtctg ctttgtttgg tggcgccaat tttaatcaaa gtgggaatat
     3001 tgctgatagc tcattgtcct tcactttcac taacagtagc aacggtccga acctcataac
     3061 aactcaaaca aattctcaag cgctttcaca accaattgcc tcctctaacg ttcatgataa
     3121 cttcatgaat aatgaaatca cggctagtaa aattgatgat ggtaataatt caaaaccact
     3181 gtcacctggt tggacggacc aaactgcgta taacgcgttt ggaatcacta cagggatgtt
     3241 taataccact acaatggatg atgtatataa ctatctattc gatgatgaag ataccccacc
     3301 aaacccaaaa aaagagtaag acccagcttt cttgtacaaa gttggcatta taagaaagca
     3361 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     3421 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     3481 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa taatatcatc
     3541 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     3601 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     3661 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     3721 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     3781 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     3841 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     3901 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     3961 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     4021 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     4081 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     4141 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     4201 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     4261 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     4321 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     4381 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     4441 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     4501 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4561 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4621 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4681 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4741 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4801 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4861 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4921 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4981 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5041 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5101 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5161 gttcctggcc ttttgctggc cttttgctca catgtt
//
