LOCUS       sequence-84521-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pREP with a mutation in the ATPase domain (K219H). This is predicted
            to result in loss of geminivirus replication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pREPK219H
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggcggccgc tctagaacta gttaattaag aattatcgaa ccactttgta caagaaagct
       61 gggtcgaatt caggcacgtt cagtgactcg acgattcccg agcaaaaaaa gtctccccgt
      121 cacacatgta gtgggtgacg caattatctt taaagtaatc cttctgttga cttgtcattg
      181 ataacatcca gtcttcgtca ggattgcaaa gaattataga agggatccca ccttttattt
      241 tcttcttttt tccatattta gggttgacag tgaaatcaga ctggcaacct attaattgct
      301 tccacaatgg gacgaacttg aaggggatgt cgtcgatgat attataggtg gcgtgttcat
      361 cgtagttggt gaaatcgatg gtaccgttcc aatagttgtg tcgtccgaga cttctagccc
      421 aggtggtatg tccggtacga gttggtccgc agatgtagag gctggggtgt cggattccat
      481 tccttccatt gtcctggtta aatcggccat ccattcaagg tcagattgag cttgttggta
      541 tgagacagga tgtatgtaag tataagcgtc tatgcttaca tggtatagat gggtttccct
      601 ccaggagtgt agatcttcgt ggcagcgaag atctgattct gtgaagggcg acacatacgg
      661 ttcaggttgt ggagggaata atttgttggc tgaatattcc agccattgaa gctttgttgc
      721 ccattcatga gggaattctt ccttgatcat gtcaagatat tcctccttag acgttgcagt
      781 ctggataata gttctccatc gtgcgtcaga tttgcgagga gagaccttat gatctcggaa
      841 atctcctctg g
//
