LOCUS       sequence-84522-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pREP with a mutation in the ATPase domain (K219H). This is predicted
            to result in loss of geminivirus replication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pREPK219H
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(8..260)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    324..348
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
       61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      241 tgccaaatgt ttgaacgatc ggggaaattc gagctccacc gcggtggcgg ccgctctaga
      301 actagttaat taagaattat cgaaccactt tgtacaagaa agctgggtcg aattcaggca
      361 cgttcagtga ctcgacgatt cccgagcaaa aaaagtctcc ccgtcacaca tgtagtgggt
      421 gacgcaatta tctttaaagt aatccttctg ttgacttgtc attgataaca tccagtcttc
      481 gtcaggattg caaagaatta tagaagggat cccacctttt attttcttct tttttccata
      541 tttagggttg acagtgaaat cagactggca acctattaat tgcttccaca atgggacgaa
      601 cttgaagggg atgtcgtcga tgatattata ggtggcgtgt tcatcgtagt tggtgaaatc
      661 gatggtaccg ttccaatagt tgtgtcgtcc gagacttcta gcccaggtgg tatgtccggt
      721 acgagttggt ccgcagatgt agaggctggg gtgtcggatt ccattccttc cattgtcctg
      781 gttaaatcgg ccatccattc aaggtcagat tgagcttgtt ggtatgagac aggatgtatg
      841 taagtataag cgtctatgct tacatggtat agatgggttt ccctccagga gtgtagatct
      901 tcgtggcagc gaa
//
