LOCUS       sequence-84523-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pREP with a mutation in the ATPase domain (K219H). This is predicted
            to result in loss of geminivirus replication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pREPK219H
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        414..758
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     702..722
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    818..842
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgcctgcagg tcaacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat
       61 acagtctcag aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac
      121 ctcctcggat tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa
      181 ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct
      241 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac
      301 gttccaacca cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca
      361 cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact
      421 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac
      481 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
      541 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
      601 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
      661 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
      721 tcctctatat aaggaagttc atttcatttg gagaggacct cgactctaga ggatccccgg
      781 gtaccgggcc ccccctcgag gcgcgccaag ctatcaaaca agtttgtaca aaaaagcagg
      841 ctccgaattc atgccttctg ctagt
//
