LOCUS       sequence-84562-e        2487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI dy B-C
FEATURES             Location/Qualifiers
     source          1..2487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(275..280,281..297,298..303)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             492..1025
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     1112..1130
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1168..1190)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1290..1309
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1389..1661)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHAGLCSRRARSQVLPEETKGIRYLDAFARYRARIHWP
                     SFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA"
     primer_bind     1503..1525
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1517..1534
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1518..1534
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1657..1673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1657..1673)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1670..1692)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1681..1697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1705..1711,1712..1729,1730..1735))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1750..1771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1888..1905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2059..2487,1..160))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2139..2158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
       61 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      121 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      181 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaggcg
      241 cacaccgtgg aaacggatga aggcacgaac ccagttgaca taagcctgtt cggttcgtaa
      301 actgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
      361 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc
      421 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
      481 gagcagcaac gatgttacgc agcagcaacg atgttacgca gcagggcagt cgccctaaaa
      541 caaagttagg tggctcaagt atgggcatca ttcgcacatg taggctcggc cctgaccaag
      601 tcaaatccat gcgggctgct cttgatcttt tcggtcgtga gttcggcgac gtagccacct
      661 actcccaaca tcagccggac tccgattacc tcgggaactt gctccgtagt aagacattca
      721 tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc tctcgcggct tacgttctgc
      781 ccaggtttga gcagccgcgt agtgagatct atatctatga tctcgcagtc tccggcgagc
      841 accggaggca gggcattgcc accgcgctca tcaatctcct caagcatgag gccaacgcgc
      901 ttggtgctta tgtgatctac gtgcaagcag attacggtga cgatcccgca gtggctctct
      961 atacaaagtt gggcatacgg gaagaagtga tgcactttga tatcgaccca agtaccgcca
     1021 cctaacaatt cgttcaagcc gagatcggct tcccggccgc ggagttgttc ggtaaattgt
     1081 cacaacgccg ctcatgacat taacctataa aaataggcgt atcacgaggc ccttttgtct
     1141 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agaaggtcac
     1201 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     1261 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     1321 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     1381 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     1441 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     1501 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta cctcgcgaat
     1561 gcatctagat atcggatccc tttggtctct tctggaagca cctgagaccg ggcccgtcga
     1621 ctgcagaggc ctgcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1681 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1741 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1801 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1861 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2461 gttcttgaag tggtggccta actacgg
//
