LOCUS       sequence-84565-e        2500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI dy F-G
FEATURES             Location/Qualifiers
     source          1..2500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             115..648
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     735..753
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(791..813)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     913..932
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1126..1148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1140..1157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1141..1157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1293..1309)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1293..1309)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1306..1328)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1317..1333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1341..1347,1348..1365,1366..1371))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1386..1407
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1524..1541)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1695..2283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1775..1794)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(2398..2403,2404..2420,2421..2426)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca
       61 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
      121 cgcagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aggtggctca
      181 agtatgggca tcattcgcac atgtaggctc ggccctgacc aagtcaaatc catgcgggct
      241 gctcttgatc ttttcggtcg tgagttcggc gacgtagcca cctactccca acatcagccg
      301 gactccgatt acctcgggaa cttgctccgt agtaagacat tcatcgcgct tgctgccttc
      361 gaccaagaag cggttgttgg cgctctcgcg gcttacgttc tgcccaggtt tgagcagccg
      421 cgtagtgaga tctatatcta tgatctcgca gtctccggcg agcaccggag gcagggcatt
      481 gccaccgcgc tcatcaatct cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc
      541 tacgtgcaag cagattacgg tgacgatccc gcagtggctc tctatacaaa gttgggcata
      601 cgggaagaag tgatgcactt tgatatcgac ccaagtaccg ccacctaaca attcgttcaa
      661 gccgagatcg gcttcccggc cgcggagttg ttcggtaaat tgtcacaacg ccgctcatga
      721 cattaaccta taaaaatagg cgtatcacga ggcccttttg tctcgcgcgt ttcggtgatg
      781 acggtgaaaa cctctgacac atgcagctcc cggagaaggt cacagcttgt ctgtaagcgg
      841 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
      901 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
      961 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     1021 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     1081 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     1141 gtaaaacgac ggccagtgaa ttcgagctcg gtacctcgcg aatgcatcta gatatcggat
     1201 cccttggtct cttgagtgac ctaagctagc ctagtgtctg agaccgggcc cgtcgactgc
     1261 agaggcctgc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1321 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1381 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1441 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1501 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1561 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1621 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1681 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1741 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1801 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1861 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1921 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1981 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2041 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2101 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2161 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2281 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2341 taagggattt tggtcatgag gcgcacaccg tggaaacgga tgaaggcacg aacccagttg
     2401 acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg
     2461 tccagaacct tgaccgaacg cagcggtggt aacggcgcag
//
