LOCUS       sequence-84600-e        2560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LII ins 4-5
FEATURES             Location/Qualifiers
     source          1..2560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     184..206
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     198..215
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     199..215
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     226..245
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        226..244
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(609..626)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(719..1307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(799..818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1478..2269,2270..2338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2101..2120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2339..2443)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccttataaat caaaagaata gaccgagata gggttgagtg gccgctacag ggcgctccca
       61 ttcgccattc aggctgcgca actgttggga agggcgtttc ggtgcgggcc tcttcgctat
      121 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      181 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgacgtaata cgactcacta
      241 tagggcgaat tggcggaagg ccgtcaaggc cacgtgtctt gtccaggcgc gccatgagct
      301 ctaaagcttc gctcgagctt ttgaagacaa tgtctagata attaggctaa actatcctta
      361 agggaatata tatatattcg aatatatata tattctgagt tgtcttccct cgaattccca
      421 agcttatctc gacccggggt agcggccgct tggatcccag aattctaggt acctcttaat
      481 taatggagca caagactggc ctcatgggcc ttccgctcac tgcccgcttt ccagtcggga
      541 aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc
      601 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct
      661 aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      781 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      841 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      901 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      961 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1021 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1081 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1141 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1201 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1261 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1321 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1381 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1441 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1501 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1561 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1621 aaccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1681 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1741 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1801 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1861 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1921 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1981 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2041 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2101 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2161 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2221 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2281 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2341 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2401 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2461 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     2521 cagctcattt tttaaccaat aggccgaaat cggcaaaatc
//
