LOCUS       sequence-84626-e        3319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI A-B 35S c2
FEATURES             Location/Qualifiers
     source          1..3319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     325..344
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     578..595
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    712..733
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(748..753,754..771,772..778)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    786..802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     791..813
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     810..826
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     810..826
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1384..1729
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1672..1692
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(1781..1798)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1781..1797)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1790..1812)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2006..2025)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2125..2147
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2185..2203)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(2290..2823)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3012..3017,3018..3034,3035..3040))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      join(3155..3319,1..424)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
       61 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      121 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      181 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      241 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      301 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      361 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      421 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      601 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      661 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      721 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      781 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      841 gcttgcatgc aggcctctgc agtcgacggg cccgggatcc gatggggtct ctgcggagat
      901 tagccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg cttacgcagc
      961 acgactcatc aagacgatct acccgagcaa taatctccag gaaatcaaat accttcccaa
     1021 gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca cagagaaaga
     1081 tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct tgcttcacaa
     1141 accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg aatcaaaggc
     1201 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
     1261 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
     1321 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag agcaaagggc
     1381 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
     1441 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
     1501 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
     1561 acccccaccc acgaggagca tcgtggaaaa agaacacgtt ccaaccacga cttcaaagca
     1621 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
     1681 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gtctgtgaga
     1741 ccccatctag atgcattcgc gaggtaccga gctcgaattc actggccgtc gttttacaac
     1801 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     1861 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     1921 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     1981 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     2041 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     2101 cttacagaca agctgtgacc ttctccggga gctgcatgtg tcagaggttt tcaccgtcat
     2161 caccgaaacg cgcgagacaa aagggcctcg tgatacgcct atttttatag gttaatgtca
     2221 tgagcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga tctcggcttg
     2281 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
     2341 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat
     2401 cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat
     2461 gccctgcctc cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg
     2521 ctgctcaaac ctgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa
     2581 ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg
     2641 ctgatgttgg gagtaggtgg ctacgtcgcc gaactcacga ccgaaaagat caagagcagc
     2701 ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga
     2761 gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa
     2821 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     2881 atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg
     2941 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     3001 acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc
     3061 gtttccacgg tgtgcgcctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3181 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3301 ttcttctagt gtagccgta
//
