LOCUS       sequence-84648-e        2537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI D-E C-NLS c2
FEATURES             Location/Qualifiers
     source          1..2537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     606..624
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(662..684)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     784..803
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     997..1019
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1011..1028
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1012..1028
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1087..1107)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1201..1217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1201..1217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1214..1236)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1225..1241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1249..1255,1256..1273,1274..1279))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1294..1315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1432..1449)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1603..2191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1683..1702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(2306..2311,2312..2328,2329..2334)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             join(2523..2537,1..519)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc aagtatgggc
       61 atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc tgctcttgat
      121 cttttcggtc gtgagttcgg cgacgtagcc acctactccc aacatcagcc ggactccgat
      181 tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt cgaccaagaa
      241 gcggttgttg gcgctctcgc ggcttacgtt ctgcccaggt ttgagcagcc gcgtagtgag
      301 atctatatct atgatctcgc agtctccggc gagcaccgga ggcagggcat tgccaccgcg
      361 ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa
      421 gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat acgggaagaa
      481 gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca agccgagatc
      541 ggcttcccgg ccgcggagtt gttcggtaaa ttgtcacaac gccgctcatg acattaacct
      601 ataaaaatag gcgtatcacg aggccctttt gtctcgcgcg tttcggtgat gacggtgaaa
      661 acctctgaca catgcagctc ccggagaagg tcacagcttg tctgtaagcg gatgccggga
      721 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
      781 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
      841 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
      901 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      961 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     1021 cggccagtga attcgagctc ggtacctcgt aggtctctga ttttacgcac tcgagtcgac
     1081 tcctccaacc tttctcttct tcttaggctg cagtcccttt gagaccatcg aatgcatcta
     1141 gatatcggat cccgggcccg tcgactgcag aggcctgcat gcaagcttgg cgtaatcatg
     1201 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1261 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1321 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1381 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1441 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1501 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1561 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1621 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1681 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1741 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1801 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1861 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1921 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1981 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2041 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2101 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2161 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2221 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaggc gcacaccgtg
     2281 gaaacggatg aaggcacgaa cccagttgac ataagcctgt tcggttcgta aactgtaatg
     2341 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2401 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagt ctatgcctcg
     2461 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     2521 cgatgttacg cagcagc
//
