LOCUS       sequence-84660-e        2734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI E-F 35S term c1
FEATURES             Location/Qualifiers
     source          1..2734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(707..712,713..729,730..735))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      850..1438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1339..1358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1592..1609
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1726..1747
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1762..1767,1768..1785,1786..1792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1800..1816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1805..1827
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1824..1840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1824..1840
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2210..2227)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2210..2226)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2219..2241)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2435..2454)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2554..2576
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2614..2632)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(join(2719..2734,1..518))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
       61 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
      121 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
      181 actgcgagat catagatata gatctcacta cgcggctgct caaacctggg cagaacgtaa
      241 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
      301 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
      361 tcgccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
      421 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
      481 ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa
      541 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa
      601 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
      661 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga
      721 acaggcttat gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gcctcatgac
      781 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      841 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      901 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      961 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1021 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1081 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1141 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1201 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1261 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1321 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1381 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1441 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1501 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1561 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1621 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1681 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     1741 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     1801 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
     1861 atgcaggcct ctgcagtcga cgggcccggg atccgatatc tagatgcatt cgatgaagac
     1921 tttacgggtc tcaaatccgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca
     1981 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
     2041 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     2101 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
     2161 ccttgagtga gacctctgaa gtcttcatcg aggtaccgag ctcgaattca ctggccgtcg
     2221 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     2281 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2341 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     2401 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     2461 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     2521 cggcatccgc ttacagacaa gctgtgacct tctccgggag ctgcatgtgt cagaggtttt
     2581 caccgtcatc accgaaacgc gcgagacaaa agggcctcgt gatacgccta tttttatagg
     2641 ttaatgtcat gagcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat
     2701 ctcggcttga acgaattgtt aggtggcggt actt
//
