LOCUS       sequence-84662-e        2733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI B-C N-NES KOZ a c1
FEATURES             Location/Qualifiers
     source          1..2733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(515..520,521..537,538..543))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      658..1246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1147..1166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1400..1417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1534..1555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1570..1575,1576..1593,1594..1600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1608..1624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1613..1635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1632..1648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1632..1648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2017..2034)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2017..2033)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2026..2048)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2242..2261)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2361..2383
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2421..2439)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(join(2526..2733,1..326))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tagatataga tctcactacg cggctgctca aacctgggca gaacgtaagc cgcgagagcg
       61 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag
      121 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc gccgaactca
      181 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat
      241 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc
      301 gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
      361 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
      421 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      481 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
      541 caactgggtt cgtgccttca tccgtttcca cggtgtgcgc ctcatgacca aaatccctta
      601 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
      661 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
      721 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
      781 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
      841 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
      901 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
      961 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1021 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1081 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1141 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1201 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1261 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1321 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     1381 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1441 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1501 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     1561 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     1621 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcaggcctct
     1681 gcagtcgacg ggcccgggat ccgatatcta gatgcattcg atggtctcat ctgaacaatg
     1741 gcaaattgtt gctctcacgg acgggactca gccgataacg gcgacgcatt ggaaaacgga
     1801 gcttccgctt caaatgctgc caattcgact ggtccaaccg cggaagcttc tgtaccacaa
     1861 tcaaaacatg cacctccttc tcctcctcct gcaaccaaac aaggccctat aggcggccgc
     1921 atgttgcaga acgagcttgc tcttaagttg gctggacttg atattaacaa gactggagga
     1981 ggcaccagag acctacgagg taccgagctc gaattcactg gccgtcgttt tacaacgtcg
     2041 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     2101 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     2161 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2221 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     2281 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     2341 cagacaagct gtgaccttct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     2401 gaaacgcgcg agacaaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgag
     2461 cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg
     2521 aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc
     2581 aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca
     2641 taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc
     2701 tgcctccggt gctcgccgga gactgcgaga tca
//
