LOCUS       sequence-84670-e        3180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI D-E 1-10GFP
FEATURES             Location/Qualifiers
     source          1..3180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     149..167
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(205..227)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     327..346
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     540..562
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     554..571
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     555..571
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1387..1403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1387..1403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1400..1422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1411..1427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1435..1441,1442..1459,1460..1465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1480..1501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1618..1635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1789..2377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1869..1888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(2492..2497,2498..2514,2515..2520)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             join(2709..3180,1..62)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 caaagttggg catacgggaa gaagtgatgc actttgatat cgacccaagt accgccacct
       61 aacaattcgt tcaagccgag atcggcttcc cggccgcgga gttgttcggt aaattgtcac
      121 aacgccgctc atgacattaa cctataaaaa taggcgtatc acgaggccct tttgtctcgc
      181 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga aggtcacagc
      241 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      301 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      361 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      421 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      481 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      541 ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtacct cgcgaatgca
      601 tctagatatc ggatcccggg cccgtcgact gcagaggtac gggtctcaaa gggaggtgga
      661 ggaggttctg gaggcggtgg aagtggtggc ggaggtagcg tgtctaaggg cgaagaactc
      721 ttcactggtg ttgtgcctat cctcgtggaa ctcgacggcg acgttaacgg tcataagttc
      781 tccgtcaggg gagagggaga aggcgacgct accatcggaa agctcacact caagttcatc
      841 tgcaccacag gcaagctccc tgtgccttgg cctactctcg ttactaccct cacctacggt
      901 gttcagtgct tctctcgtta ccctgaccac atgaagcagc atgacttctt caagagtgct
      961 atgcctgagg gctacgtcca ggaacgtaca atctctttca aggacgacgg aaagtacaag
     1021 acaagggccg tcgtgaagtt cgagggtgac actctcgtga accgtatcga actcaaggga
     1081 accgacttca aggaggacgg taacatcctc ggtcacaagc tcgaatacaa cttcaactcc
     1141 cataacgtgt acatcacagc tgacaagcag aagaacggaa tcaaggccaa cttcactgtt
     1201 aggcacaacg tcgaggacgg ttctgttcag ctcgccgacc attaccagca gaacacccct
     1261 atcggtgacg gacctgtcct cctccctgac aaccactacc tctctacaca gactgtgctc
     1321 tccaaggacc ctaacgaaaa gtaaaatctg agacctctgc ctgcatgcaa gcttggcgta
     1381 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1441 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     1501 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1561 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1621 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1681 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1741 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1801 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1861 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1921 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1981 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2041 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2101 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2161 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2221 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2281 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2341 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2401 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgaggcgcac
     2461 accgtggaaa cggatgaagg cacgaaccca gttgacataa gcctgttcgg ttcgtaaact
     2521 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2581 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat
     2641 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     2701 cagcaacgat gttacgcagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2761 agttaggtgg ctcaagtatg ggcatcattc gcacatgtag gctcggccct gaccaagtca
     2821 aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt cggcgacgta gccacctact
     2881 cccaacatca gccggactcc gattacctcg ggaacttgct ccgtagtaag acattcatcg
     2941 cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct cgcggcttac gttctgccca
     3001 ggtttgagca gccgcgtagt gagatctata tctatgatct cgcagtctcc ggcgagcacc
     3061 ggaggcaggg cattgccacc gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg
     3121 gtgcttatgt gatctacgtg caagcagatt acggtgacga tcccgcagtg gctctctata
//
