LOCUS       sequence-84681-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed N-terminally Hexahistine tagged TRF2 Delta Basic protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Bower BD, Griffith JD
  TITLE     TRF1 and TRF2 differentially modulate Rad51-mediated telomeric and 
            nontelomeric displacement loop formation in vitro.
  JOURNAL   Biochemistry. 2014 Sep 2;53(34):5485-95. doi: 10.1021/bi5006249. 
            Epub 2014 Aug 21.
  PUBMED    25115914
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi5006249";
            journalName: "Biochemistry"; date: "2014-09-2- 2"; volume: "53"; 
            issue: "34"; pages: "5485-95"
COMMENT     Sequence Label: pTRCHisB-TRF2∆B
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             101..127
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             164..196
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             209..229
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agcggcactg ctctttaaca atttatcaga caatctgtgt gggcactcga ccggaattat
       61 cgattaactt tattattaaa aattaaagag gtatatatta atgtatcgat taaataagga
      121 ggaataaacc atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg
      181 tggacagcaa atgggtcggg atctgtacga aaacctgtat tttcagggcg atccgagctc
      241 gagatctgcg cgtgatcgat cgcggctgga agaggcagtc aatcgctggg tgctcaagtt
      301 ctacttccac gaggcgctgc gggcctttcg gggtagccgg tacggggact tcagacagat
      361 ccgggacatc atgcaggctt tgcttgtcag gcccttgggg aaggagcaca ccgtgtcccg
      421 attgctgcgg gttatgcagt gtctgtcgcg gattgaagaa ggggaaaatt tagactgttc
      481 ctttgatatg gaggctgagc tcacaccact ggaatcagct atcaatgtgc tggagatgat
      541 taaaacggaa tttacactga cagaagcagt ggtcgaatcc agtagaaaac tggtcaagga
      601 agctgctgtc attatttgta tcaaaaacaa agaatttgaa aaggcttcaa aaattttgaa
      661 aaaacatatg tccaaggacc ccacaactca gaagctgaga aatgatctcc tgaatattat
      721 tcgagaaaag aacttggccc atcctgttat ccagaacttt tcatatgaga ccttccagca
      781 gaagatgctg cgcttcctgg agagccacct ggatgacgcc gagccctacc tcctcacgat
      841 ggccaaaaag gctttgaaat ctgagtccgc tgcctcaagt acagggaa
//
