LOCUS       sequence-84687-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive mammalian expression of ERAS. Upon CRE mediated 
            deletion of ERAS insert, dsRED expression markers is truned on due 
            to concomittant removal of stop cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRE...
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    157..190
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
       61 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga
      121 actccttgat gacgtcctcg gaggaggcca tggtggataa cttcgtataa tgtatgctat
      181 acgaagttat acctaagtaa ctagtgcggc caattcgccc ttcgtcgacg tcaccctggc
      241 ctggggaaag gtctacattt cttggccaag accttcaggc cacagagcag ccacagtggc
      301 aggtggcctt ctggtgccgg gtcttctccc tacaggacct tgccatgggc tccttcgcca
      361 tggcctcctg gaccctctgg atctcatgga ccagcaggga aaaggcctcc tccacgcctt
      421 gccgtgtttt ggccgaggtc tccacgaagt gggcccccca gctgtgtgcg agggctgcag
      481 cagcggcatg agcatctcca gcagtggtca caaggtcaca cttgttgccc acgaggacaa
      541 ggggctgggc ggggtgaggg ccccaggtgg cccatatctg ctgcagctgg atcagagacg
      601 aggggtcatc gagagcgaag acgcccagca caccatcaca gacagccagg cactggtcac
      661 gcagggccct atggatggcc tgccctgctg tgtccagcac attcagaatg cagtccccac
      721 tgtccagggt caactccttc cagtaggaat cctggatggt ggggtcgtgg tcctccacga
      781 a
//
