LOCUS       sequence-84689-e        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL5 and attL2 sites flanking us:NPTII 
            repair template sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB98
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..38
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     30..47
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     31..47
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    62..157
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
       61 ccaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
      121 tgctttttta taatgccaac tttgtataca aaagttgccg agctcgcggc cgcagcagtc
      181 ttacttccat gatttcttta actatgccgg aatccatcgc agcgtaatgc tctacaccac
      241 gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca
      301 cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc
      361 ggatcaacag gtggttgcaa ctggacaagg cactagcggg actttgcaag tggtgaatcc
      421 gcacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag ccaaaagcca
      481 gacagagtgt gatatctacc cgcttcgcgt cggcatccgg tcagtggcag tgaagggcga
      541 acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc atgaagatgc
      601 ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg cacgaccacg cattaatgga
      661 ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag agatgctcga
      721 ctgggcagat gaacatggca tcgtggtgat tgatgaaact gctgctgtcg gctttaacct
      781 ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc
      841 agtcaacggg gaaactcagc aagcgcactt acaggcgatt aaagagctga tagcgcgtga
      901 caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata cccgtccgca
      961 aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg acccgacgcg
     1021 tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca tcagcgatct
     1081 ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaa
//
