LOCUS       sequence-84690-e        1121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL5 and attL2 sites flanking us:NPTII 
            repair template sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB98
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     157..173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     157..173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        178..196
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    214..313
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        360..378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(403..655)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(793..812)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggtagcag cgaagtcgag
       61 gcatttctgt cctggctggt ccagaggagg catgatgata tatttttatc ttgtgcaatg
      121 taacatcaga gattttgaga cacgggccag agctgccagg aaacagctat gaccatgtaa
      181 tacgactcac tataggggat atcagctgga tggcaaataa tgattttatt ttgactgata
      241 gtgacctgtt cgttgcaaca aattgatgag caattatttt ttataatgcc aactttgtac
      301 aagaaagctg ggtcgtcgac ctgcagcccc gcgcgttggc cgattcatta atgcaggttt
      361 aatacgactc actatagggc gaatggaact ggacgggccc acgatctagt aacatagatg
      421 acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt
      481 aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt
      541 acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa
      601 gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga
      661 aattcgagct ctccgcggtt atcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
      721 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
      781 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
      841 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
      901 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcattctcg ccttgagcct
      961 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     1021 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     1081 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt g
//
