LOCUS       sequence-84691-e        1191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI
            coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB99
FEATURES             Location/Qualifiers
     source          1..1191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(25..148)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(193..445)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(459..1043)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
                     EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
                     VEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
                     VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ctatagggga tatcagctgg atggcatagt gactggatat gttgtgtttt acagtattat
       61 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      121 tctcgttcaa cttttgtata caaagttgcg aattcgccct ttccggaagc tgtcgagggg
      181 ggatcaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
      241 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
      301 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
      361 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
      421 tttattgcca aatgtttgaa cgatcggacg tcctattaaa agtttatctc accgttatta
      481 aatttccttc tcacttcctc aagtgtcaat gtaccagctt taatcatttc tccaccaatc
      541 aaaagctctt ctacactaag aacagctcca ttacaattag tgatatgatt caatcttgta
      601 agctgagctt tgtagtttcc tttgaaatga ccactcacaa acaagaactt aaattctgtt
      661 actgaagatg gatagacttt ccaccattca ttagggttga tatgcttgtt tcttgtttga
      721 ttctcttcga catatctttg catttcatct gcttgaccaa ttggaagatt ataacctcct
      781 gaatatgcct tagtatcaac gatcacaccg taatcaatag gagatccgac agtataaatt
      841 gctccgtctg gtttccttga tccacccaaa tgtttaccac gataaccata aaccttcata
      901 aagaactcca tcaccttcat ttcaaggatt ctatcctgag ttgaatttct tgcgatttca
      961 atcaattcaa tatattcatg tggcacatat ttcaatttat gtctaagttc agatttcttc
     1021 tcttccaatt cagatttcac tagttccttc tgacgaagat ggatctttgt atgacgcttc
     1081 ctttcatcac ttcttgcgaa cttacgacca cagatgtcac atgcgaatgg tttctcacca
     1141 gtatgggttc tgatatgtgt agtcaaatga tcgctacgac taaagttcct c
//
