LOCUS       sequence-84693-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO 
            knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 
            31939).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: FOR human OCT4 TALEN
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..74
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             75..101
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 aagatattgt atatatcgta acaataggag gttcaacaat ggcttcctcc cctccaaaga
       61 aaaagagaaa ggttagttgg aaggacgcaa gtggttggtc tagaatgcat gcggatccca
      121 ttcgtccgcg caggccaagt cctgcccgcg agcttctgcc cggaccccaa ccggataggg
      181 ttcagccgac tgcagatcgt ggggtgtctg cgcctgctgg cagccctctg gatggcttgc
      241 ccgctcggcg gacggtgtcc cggacccggc tgccatctcc ccctgcgccc tcacctgcgt
      301 tctcggcggg cagcttcagc gatctgctcc gtccgttcga tccgtcgctt cttgatacat
      361 cgcttcttga ttcgatgcct gccgtcggca cgccgcatac agcggctgcc ccagcagagt
      421 gggatgaggc gcaatcggct ctgcgtgcag ccgatgaccc gccacccacc gtgcgtgtcg
      481 ctgtcactgc cgcgcggccg ccgcgcgcca agccggcccc gcgacggcgt gctgcgcaac
      541 cctccgacgc ttcgccggcc gcgcaggtgg atctacgcac gctcggctac agtcagcagc
      601 agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac gaggcactgg
      661 tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacc
//
