LOCUS       sequence-84694-e        1121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI
            coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pNJB99
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..117
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             151..171
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             442..1026
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
                     EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
                     VEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
                     VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctccg
      121 aattcgccct tcaccatggc ttcctcccct ccaaagaaaa agagaaaggt tcttgaattg
      181 ccttacgctt gtccagttga gtcttgtgat agacgtttta gtaggtcaga cgaactcact
      241 aggcatatta gaatccacac aggacagaag cctttccagt gtagaatctg tatgaggaac
      301 tttagtcgta gcgatcattt gactacacat atcagaaccc atactggtga gaaaccattc
      361 gcatgtgaca tctgtggtcg taagttcgca agaagtgatg aaaggaagcg tcatacaaag
      421 atccatcttc gtcagaagga actagtgaaa tctgaattgg aagagaagaa atctgaactt
      481 agacataaat tgaaatatgt gccacatgaa tatattgaat tgattgaaat cgcaagaaat
      541 tcaactcagg atagaatcct tgaaatgaag gtgatggagt tctttatgaa ggtttatggt
      601 tatcgtggta aacatttggg tggatcaagg aaaccagacg gagcaattta tactgtcgga
      661 tctcctattg attacggtgt gatcgttgat actaaggcat attcaggagg ttataatctt
      721 ccaattggtc aagcagatga aatgcaaaga tatgtcgaag agaatcaaac aagaaacaag
      781 catatcaacc ctaatgaatg gtggaaagtc tatccatctt cagtaacaga atttaagttc
      841 ttgtttgtga gtggtcattt caaaggaaac tacaaagctc agcttacaag attgaatcat
      901 atcactaatt gtaatggagc tgttcttagt gtagaagagc ttttgattgg tggagaaatg
      961 attaaagctg gtacattgac acttgaggaa gtgagaagga aatttaataa cggtgagata
     1021 aacttttaat aggacgtccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     1081 tcctgttgcc ggtcttgcga tgattatcat ataatttctg t
//
