LOCUS       sequence-84700-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed N-terminally Hexahistidine tagged TRF1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Bower BD, Griffith JD
  TITLE     TRF1 and TRF2 differentially modulate Rad51-mediated telomeric and 
            nontelomeric displacement loop formation in vitro.
  JOURNAL   Biochemistry. 2014 Sep 2;53(34):5485-95. doi: 10.1021/bi5006249. 
            Epub 2014 Aug 21.
  PUBMED    25115914
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi5006249";
            journalName: "Biochemistry"; date: "2014-09-2- 2"; volume: "53"; 
            issue: "34"; pages: "5485-95"
COMMENT     Sequence Label: pTrcHisB-TRF1
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             99..125
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             141..158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             162..194
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             207..227
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cggcactgct ctttaacaat ttatcagaca atctgtgtgg gcactcgacc ggaattatcg
       61 attaacttta ttattaaaaa ttaaagaggt atatattaat gtatcgatta aataaggagg
      121 aataaaccat ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg
      181 gacagcaaat gggtcgggat ctgtacgaaa acctgtattt tcagggcgat ccgagctcga
      241 gatccgcgcg tgatcgatcg gaggatgttt cctcagcggc cccgagcccg cggggctgtg
      301 cggatggtag ggatgccgac cctactgagg agcagatggc agaaacagag agaaacgacg
      361 aggagcagtt cgaatgccag gaactgctcg agtgccaggt gcaggtgggg gcccccgagg
      421 aggaggagga ggaggaggag gacgcgggcc tggtggccga ggccgaggcc gtggctgccg
      481 gctggatgct cgatttcctc tgcctctctc tttgccgagc tttccgcgac ggccgctccg
      541 aggacttccg caggacccgc aacagcgcag aggctattat tcatggacta tccagtctaa
      601 cagcttgcca gttgagaacg atatacatat gtcagttttt gacaagaatt gcagcaggaa
      661 aaacccttga tgcacagttt gaaaatgatg aacgaattac acccttggaa tcagccctga
      721 tgatttgggg ttcaattgaa aaggaacatg acaaacttca tgaagaaata cagaatttaa
      781 ttaaaattca ggctatagct gtttgtatgg aaaatggcaa ctttaaagaa gcagaagaag
      841 tctttgaaag aatatttggt gatccaaatt ctcatatgcc tttcaaaagc aaattgctta
      901 tgataatctc tca
//
