LOCUS       sequence-84734-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter control for pMPRA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
  TITLE     Massively parallel reporter assays in cultured Mammalian cells.
  JOURNAL   J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
  PUBMED    25177895
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-08-17"; volume: "(90). doi"; pages: " 
            10.3791/51719"
COMMENT     Sequence Label: pMPRA3-CMV-Clover
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        57..414
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        57..248
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(80..100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      265..400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     327..346
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      439..448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcgcagcac catggcctga aataacctct gaaagaggaa cttggttagc taccttctga
       61 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
      181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      301 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
      361 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
      421 gattcttctg acactagcgc caccatggca ccctgcatgc tgctcctgct gttggcggct
      481 gccctggccc cgactcagac ccgcgcgggc ccacattcgc tgaggtattt ccacaccgcc
      541 gtgtcccggc ccggcctcgg gaagccccgg ttcatctctg tcggctacgt ggacgacacg
      601 cagttcgtgc gcttcgacag cgacgcggag aatccgaggt atgagccgcg ggtgcggtgg
      661 atggagcagg tggagcccga gtattgggag cggaacacgc agatcgccaa gggcaatgag
      721 cagattt
//
