LOCUS       sequence-84736-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter control for pMPRA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
  TITLE     Massively parallel reporter assays in cultured Mammalian cells.
  JOURNAL   J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
  PUBMED    25177895
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-08-17"; volume: "(90). doi"; pages: " 
            10.3791/51719"
COMMENT     Sequence Label: pMPRA2-CMV-Clover
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..510
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     429..447
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      511..520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aatcgataat tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
       61 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
      121 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
      181 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
      241 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
      301 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
      361 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
      421 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
      481 gttgaccgaa tcaccgacct ctctccccag gccaccatgg caccctgcat gctgctcctg
      541 ctgttggcgg ctgccctggc cccgactcag acccgcgcgg gcccacattc gctgaggtat
      601 ttccacaccg ccgtgtcccg gcccggcctc gggaagcccc ggttcatctc tgtcggctac
      661 gtggacgaca cgcagttcgt gcgcttcgac agcgacgcgg agaatccgag gtatga
//
