LOCUS       sequence-84796-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pcDNA3.0-based plasmid encoding fDHFR-UbK48R-A-beta-42 13myc under 
            the control of T7 or CMV promoter for 35S-pulse-chase URT-based 
            assays in rabbit reticulocyte extract..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Brower CS, Piatkov KI, Varshavsky A
  TITLE     Neurodegeneration-associated protein fragments as short-lived 
            substrates of the N-end rule pathway.
  JOURNAL   Mol Cell. 2013 Apr 25;50(2):161-71. doi: 
            10.1016/j.molcel.2013.02.009. Epub 2013 Mar 14.
  PUBMED    23499006
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.02.009"; journalName: "Mol Cell"; date: 
            "2013-04-25- 25"; volume: "50"; issue: "2"; pages: "161-71"
COMMENT     Sequence Label: pCB268
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..46
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     31..47
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(99..596)
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgaggtcgac ggtatcgata agcttgatat
       61 cgaattcact agtgattgat taatttttgt tcaccgttca agtcttcctc ggagattagc
      121 ttttgttcac cgttcaaatc ttcttcagaa atcaactttt gttcaccgtc gagtccgttc
      181 aagtcttctt ctgagattaa tttttgttca ccgttcaagt cttcctcgga gattagcttt
      241 tgttcaccgt tcaaatcttc ttcagaaatc aacttttgtt caccgtcgag tccgttcaag
      301 tcttcttctg agattaattt ttgttcaccg ttcaagtctt cctcggagat tagcttttgt
      361 tcaccgttca aatcttcttc agaaatcaac ttttgttcac cgtcgagtcc gttcaagtct
      421 tcttctgaga ttaatttttg ttcaccgttc aagtcttcct cggagattag cttttgttca
      481 ccgttcaaat cttcttcaga aatcaacttt tgttcaccgt cgagtccgtt caagtcttct
      541 tctgagatta atttttgttc accgttcaag tcttcctcgg agattagctt ttgttcaccg
      601 ttaattaacc cgggcgctat gacaacaccg cccaccatga gtccaatgat cgcacctttg
      661 tttgaaccca catcttcagc aaagaacacc aatttttgat gatgaacctc atatccagaa
      721 tcatgtcgga actccgcatc accaccgcgg agccttagca caagatgtaa ggtggactcc
      781 ttctgaatgt tgtaatcaga cagcgttcta ccgtcttcta gctgcctacc ggca
//
