LOCUS       sequence-84803-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty backbone for retroviral gene expression. Select recipient 
            cells using GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     Viral vectors.
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBABE GFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(49..65)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(50..66)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             71..790
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
ORIGIN
        1 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttatcg ataccgtcga
       61 cctcgagacc atggtgagca agcagatcct gaagaacacc ggcctgcagg agatcatgag
      121 cttcaaggtg aacctggagg gcgtggtgaa caaccacgtg ttcaccatgg agggctgcgg
      181 caagggcaac atcctgttcg gcaaccagct ggtgcagatc cgcgtgacca agggcgcccc
      241 cctgcccttc gccttcgaca tcctgagccc cgccttccag tacggcaacc gcaccttcac
      301 caagtacccc gaggacatca gcgacttctt catccagagc ttccccgccg gcttcgtgta
      361 cgagcgcacc ctgcgctacg aggacggcgg cctggtggag atccgcagcg acatcaacct
      421 gatcgaggag atgttcgtgt accgcgtgga gtacaagggc cgcaacttcc ccaacgacgg
      481 ccccgtgatg aagaagacca tcaccggcct gcagcccagc ttcgaggtgg tgtacatgaa
      541 cgacggcgtg ctggtgggcc aggtgatcct ggtgtaccgc ctgaacagcg gcaagttcta
      601 cagctgccac atgcgcaccc tgatgaagag caagggcgtg gtgaaggact tccccgagta
      661 ccacttcatc cagcaccgcc tggagaagac ctacgtggag gacggcggct tcgtggagca
      721 gcacgagacc gccatcgccc agctgaccag cctgggcaag cccctgggca gcctgcacga
      781 gtgggtgtaa tagggtacct tcgataaaat aaaagatttt atttagtctc
//
