LOCUS       sequence-84820-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-NtermN3
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             75..140
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             147..167
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 tggctagcac catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt
      121 acaaggatga cgatgacaag atggccccca agaagaagag gaaggtgggc attcaccgcg
      181 gggtacctat ggtggacttg aggacactcg gttattcgca acagcaacag gagaaaatca
      241 agcctaaggt caggagcacc gtcgcgcaac accacgaggc gcttgtgggg catggcttca
      301 ctcatgcgca tattgtcgcg ctttcacagc accctgcggc gcttgggacg gtggctgtca
      361 aataccaaga tatgattgcg gccctgcccg aagccacgca cgaggcaatt gtaggggtcg
      421 gtaaacagtg gtcgggagcg cgagcacttg aggcgctgct gactgtggcg ggtgagctta
      481 gggggcctcc gctccagctc gacaccgggc agctgctgca gatcgcgcag caggggggag
      541 taacagcggt agaggcagtg cacgcctggc gcaatgcgct caccggggcc cccttgaacc
      601 tgaccccaga ccaggtagtc gcaatcgcgt caaacggagg gggaaagcaa gccctggaaa
      661 ccgtgcaaag gttgttgccg gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg
      721 tggccattgc atcccacgac ggtggcaaac aggctcttga gacggttcag agacttctcc
      781 cagttctctg tcaagcccac gggctgactc ccgatcaagt tgtagcgatt gcgtcgaaca
      841 ttggagggaa acaagcattg gagactgtcc aacggctcct tcccgtgttg tgtcaagccc
      901 acggtttgac gcctgcacaa gtggtcgcca tcgcctcgaa tggcggcggt aagcag
//
