LOCUS       sequence-84822-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-NtermN3
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..68)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(84..671)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 tggtgatggt gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata
       61 ggcttacctt cgaagggccc ttaaaagttt atctcgccgt tattaaattt ccgtctcact
      121 tcctctaagg ttaatgtgcc ggctttaatc atttctccac caattaaaag ctcttctaca
      181 ctaagaacag ctccattaca attagtgata tgatttaatc gtgtaagctg agctttgtag
      241 tttcctttaa agtgaccact cacaaataaa aacttaaatt ccgttacaga agatggatag
      301 actttccacc attcattagg gttgagatgt ttgtttcgtg tttgattttc ttcgacatat
      361 cgctccattt catctgcttg gccaattggc agattataac ctccgctata agctttagta
      421 tccacgatca caccgtaatc aataggagat ccgacagtat aaattgctcc gtccggtttc
      481 cttgatccac ccaaatgttt acctctatat ccataaactt tcataaaaaa ttccattacc
      541 ttcatttcaa gaattctatc ctgagtggaa tttctggcaa tttcaattaa ttcaatatat
      601 tcatgaggca catatttcaa tttatgacga agttcagatt tcttctcctc cagttcactt
      661 ttgactagtt gggatcccgc gactcgatgg gaagttctct cgggaatccg ccggttggtt
      721 cttttgatca atgcgggagc atgaggcaga ccctttttga ctgcatcgag cgcgggtcgt
      781 ccaccaagac atgccaacgc caccagatgg tcattcgtta acgcagccaa cgcgggatcg
      841 ggcctggaca attgggctac gatggactcc aaggctggcc gtcctccccc attagaagca
      901 atggcgacca cctgttcggg tgtcagtcca tgatcctggc acagtacagg cagcaggcgc
      961 tgtactg
//
