LOCUS       sequence-84824-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Membrane tagged G-CaMP3 in cells expressing Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Vrontou S, Wong AM, Rau KK, Koerber HR, Anderson DJ
  TITLE     Genetic identification of C fibres that detect massage-like stroking
            of hairy skin in vivo.
  JOURNAL   Nature. 2013 Jan 31;493(7434):669-73. doi: 10.1038/nature11810.
  PUBMED    23364746
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2013-01-31- 31"; volume: "493"; issue: "7434"; 
            pages: "669-73"
COMMENT     Sequence Label: AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..61
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          116..591
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(704..728)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(725..824)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(754..774)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 catccatctt gttcaatcat ggtggcgata acttcgtata atgtatgcta tacgaagtta
       61 tgaattcttt gccaaagtga tgggccagca cacagaccag cacgttgccc aggagctgtg
      121 ggaggaagat aagaggtatg aacatgatta gcaaaagggc ctagcttgga ctcagaataa
      181 tccagcctta tcccaaccat aaaataaaag cagaatggta gctggattgt agctgctatt
      241 agcaatatga aacctcttac atcagttaca atttatatgc agaaatattt atatgcagaa
      301 atattgctat tgccttaacc cagaaattat cactgttatt ctttagaatg gtgcaaagag
      361 gcatgataca ttgtatcatt attgccctga aagaaagaga ttagggaaag tattagaaat
      421 aagataaaca aaaaagtata ttaaaagaag aaagcatttt ttaaaattac aaatgcaaaa
      481 ttaccctgat ttggtcaata tgtgtaccct gttacttctc cccttcctat gacatgaact
      541 taaccataga aaagaagggg aaagaaaaca tcaagggtcc catagactca ccctgaagtt
      601 ctcaggatcc gagctcggta ccacatgtaa gcttcgaggg gaggctggat cggtcccggt
      661 gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta
      721 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      781 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
      841 cattgacgtc aatggg
//
