LOCUS       sequence-84830-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Membrane tagged G-CaMP3 in cells expressing Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Vrontou S, Wong AM, Rau KK, Koerber HR, Anderson DJ
  TITLE     Genetic identification of C fibres that detect massage-like stroking
            of hairy skin in vivo.
  JOURNAL   Nature. 2013 Jan 31;493(7434):669-73. doi: 10.1038/nature11810.
  PUBMED    23364746
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2013-01-31- 31"; volume: "493"; issue: "7434"; 
            pages: "669-73"
COMMENT     Sequence Label: AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(76..97)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(310..366)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      378..387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(394..408,409..417))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(421..453)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(457..474)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      504..513
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    532..565
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    728..775
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gcccttgctc accatgctcc ctccggtacc gcccttgtac agctcgtcca tgccgagagt
       61 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      121 tttcgaaagt ttggactgca cgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      181 gccgatgggg gtgttctgct ggtagtggta ggcgagctgc acgccgccgt cctcgatgtt
      241 gtggcggatc ttgaagttcg ccttgatgcc gttcttctgc ttgtcggcct tgatatagac
      301 gttctcgagt gagctcagcc gacctatagc tctgactgcg tgacctgtct tattccactt
      361 acgacgtgat gagtcgacca tggtggcgag atccttatcg tcatcgtcgt acagatcccg
      421 acccatttgc tgtccaccag tcatgctagc cataccatga tgatgatgat gatgagaacc
      481 catggtcttg gagaagcaac aacccatggt ggcgcgccag ctgatccgac gataacttcg
      541 tataatgtat gctatacgaa gttattggca gggcctgccg ccccgacgtt ggctgcgagc
      601 cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagtaac gcgggcgtat
      661 tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg
      721 cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcata
      781 gcgcg
//
