LOCUS       sequence-84833-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 
            DNA-binding domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Guilinger JP, Thompson DB, Liu DR
  TITLE     Fusion of catalytically inactive Cas9 to FokI nuclease improves the 
            specificity of genome modification.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909.
  PUBMED    24770324
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909."
COMMENT     Sequence Label: FokI-dCas9
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caaacacgaa acaaacatat caaccctaat gaatggtgga aagtctatcc atcttctgta
       61 acggaattta agtttttatt tgtgagtggt cactttaaag gaaactacaa agctcagctt
      121 acacgattaa atcatatcac taattgtaat ggagctgttc ttagtgtaga agagctttta
      181 attggtggag aaatgattaa agccggcaca ttaaccttag aggaagtcag acggaaattt
      241 aataacggcg agataaactt tagcggcagc gagactcccg ggacctcaga gtccgccaca
      301 cccgaaagtg ataaaaagta ttctattggt ttagctatcg gcactaattc cgttggatgg
      361 gctgtcataa ccgatgaata caaagtacct tcaaagaaat ttaaggtgtt ggggaacaca
      421 gaccgtcatt cgattaaaaa gaatcttatc ggtgccctcc tattcgatag tggcgaaacg
      481 gcagaggcga ctcgcctgaa acgaaccgct cggagaaggt atacacgtcg caagaaccga
      541 atatgttact tacaagaaat ttttagcaat gagatggcca aagttgacga ttctttcttt
      601 caccgtttgg aagagtcctt ccttgtcgaa gaggacaaga aacatgaacg gcaccccatc
      661 tttggaaaca tagtagatga ggtggcatat catgaaaagt acccaacgat ttatcacctc
      721 agaaaaaagc tagttgactc aactgataaa gcggacctga ggttaatcta cttggctctt
      781 gcccatatga taaagttccg tgggcacttt ctcattgagg gtgatctaaa tccggacaac
      841 tcggatgtcg acaaactgtt catccagtta gtacaaacct ataatcagtt gtttgaagag
      901 aaccctataa atgcaagtgg cgt
//
