LOCUS       sequence-84834-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 
            DNA-binding domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Guilinger JP, Thompson DB, Liu DR
  TITLE     Fusion of catalytically inactive Cas9 to FokI nuclease improves the 
            specificity of genome modification.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909.
  PUBMED    24770324
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909."
COMMENT     Sequence Label: FokI-dCas9
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acgactcaga aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt
       61 aaagaactgg gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac
      121 gagaaacttt acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg
      181 gacataaacc gtttatctga ttacgacgtc gatgccattg taccccaatc ctttttgaag
      241 gacgattcaa tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac
      301 aatgttccaa gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat
      361 gcgaaactga taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg
      421 tctgaacttg acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca
      481 aagcatgttg cacagatact agattcccga atgaatacga aatacgacga gaacgataag
      541 ctgattcggg aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag
      601 gattttcaat tctataaagt tagggagata aataactacc accatgcgca cgacgcttat
      661 cttaatgccg tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt
      721 gtgtatggtg attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag
      781 ataggcaagg ctacagccaa atacttcttt tattctaaca ttatgaattt ctttaagacg
      841 gaaatcactc tggcaaacgg agagatacgc aaacgacctt taattgaaac caatggggag
      901 acaggtgaaa tcgtatggga taagggccgg gacttcgcga cggtgagaaa agttttgtcc
      961 atgccccaag tcaacatagt aaagaaaact g
//
