LOCUS       sequence-84847-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM
  TITLE     Sensing chromosome bi-orientation by spatial separation of aurora B 
            kinase from kinetochore substrates.
  JOURNAL   Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. 
            Epub 2009 Jan 15.
  PUBMED    19150808
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1167000"; journalName: "Science"; date: "2009-03-6-
            6"; volume: "323"; issue: "5919"; pages: "1350-3"
COMMENT     Sequence Label: Cenp-B-targeted Aurora B FRET sensor
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      615..624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttggc gcgccgccac catgggcccc aagaggcgac
      121 agctgacgtt ccgggagaag tcacggatca tccaggaggt ggaggagaat ccggacctgc
      181 gcaagggcga gatcgcgcgg cgcttcaaca tcccgccgtc cacgctgagc acgatcctga
      241 agaacaagcg cgccatcctg gcgtcggagc gcaagtacgg ggtggcctcc acctgccgca
      301 agaccaacaa gctgtctccc tacgacaagc tcgagggctt gctcatcgcc tggttccagc
      361 agatccgcgc cgccggcctg ccggtcaagg gcatcatcct caaggagaag gcgctgcgca
      421 tagccgagga gctgggcatg gacgacttca ccgcctccaa cggctggctg gaccgcttcc
      481 gccggcgcca cggcgtggtg tcctgcagcg gcgtggcccg cgcccgcgcg cgaaacgctg
      541 ccccccgcac cccggcggcg cctgccagtc cggccgcggt gccctcggag ggcagtggcg
      601 ggctagcctc gagcgccacc atggtgagca agggcgagga gaccacaatg ggcgtaatca
      661 agcccgacat gaagatcaag ctgaagatgg agggcaacgt gaatggccac gccttcgtga
      721 tcgagggcga gggcgagggc aagccctacg acggcaccaa caccatcaac ctggaggtga
      781 aggagggagc ccccctgccc ttctcctacg acattctgac ccacc
//
