LOCUS       sequence-84871-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the GalL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p415-GalL-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 actagtggat cccccgggaa aaaaatggac aagaagtact ccattgggct cgatatcggc
       61 acaaacagcg tcggttgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc
      121 aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg
      181 ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat
      241 acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag
      301 gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag
      361 cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac
      421 ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg
      481 ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg
      541 gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac
      601 aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg
      661 agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag
      721 aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt
      781 aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat
      841 gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg
      901 gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc
      961 accaaagctc cgctgagcgc tagtatgatc aagcgctatg atga
//
