LOCUS       sequence-84872-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the GalL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p415-GalL-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..111)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gattatcgaa cttccgttgt gtgatcagtt tggcgttcag cagctgccgc caataatttt
       61 tcattttctt gacaacttct tctgagggga cgttatcact cttccctcta tttttatcgg
      121 atcttgtcaa cactttatta tcaatagaat catctttgag aaaagactgg ggcacgatat
      181 gatccacgtc gtagtcggag agccgattga tgtccagttc ctgatccacg tacatgtccc
      241 tgccgttctg caggtagtac aggtagagct tctcattctg aagctgggtg ttttcaactg
      301 ggtgttcctt aaggatttgg gaccccagtt cttttatacc ctcttcaatc ctcttcatcc
      361 tttccctact gttcttctgt cccttctggg tagtttggtt ctctcgggcc atctcgataa
      421 cgatattctc gggcttatgc cttcccatta ctttgacgag ttcatccacg accttaacgg
      481 tctgcagtat tccctttttg atagctgggc tacctgcaag attagcgatg tgctcgtgaa
      541 gactgtcccc ctggccagaa acttgtgctt tctggatgtc ctccttaaag gtgagagagt
      601 catcatggat caactgcatg aagttccggt tggcaaatcc atcggactta agaaaatcca
      661 ggattgtctt tccactctgc ttgtctcgga tcccattgat cagttttctt gacagccgcc
      721 cccatcctgt atatcggcgc ctcttgagct gtttcatgac tttgtcgtcg aagagatgag
      781 cgtaagtttt caagcgttct tcaatcatct ccctatcttc aaacaacgta agggtgagga
      841 caatgtcctc aagaatgtcc tcgttctcct cattgtccag gaagtccttg tctttaatga
      901 ttttcaggag atcgtgatac gttcccagg
//
