LOCUS       sequence-84873-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the GalL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p415-GalL-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(488..507)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     607..629
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(667..685)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctctttttt tatgatattt gtacataaac tttataaatg aaattcataa tagaaacgac
       61 acgaaattac aaaatggaat atgttcatag ggtagacgaa actatatacg caatctacat
      121 acatttatca agaaggagaa aaaggaggat gtaaaggaat acaggtaagc aaattgatac
      181 taatggctca acgtgataag gaaaaagaat tgcactttaa cattaatatt gacaaggagg
      241 agggcaccac acaaaaagtt aggtgtaaca gaaaatcatg aaactatgat tcctaattta
      301 tatattggag gattttctct aaaaaaaaaa aaatacaaca aataaaaaac actcaatgac
      361 ctgaccattt gatggagttt aagtcaatac cttcttgaac catttcccat aatggtgaaa
      421 gttccctcaa gaattttact ctgtcagaaa cggccttaac gacgtagtcg atatggtgca
      481 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
      541 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
      601 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
      661 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      721 aggacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga tggaataatt
      781 tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt agaaagtaaa
      841 taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt ttaaaaaatt
      901 tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga
//
