LOCUS       sequence-84874-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the GalL promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p415-GalL-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..29)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agagatcaca ccttcctctt cttcttgggg tcagccctgc tgtctccacc gagctgagag
       61 aggtcgattc ttgtttcata gagccccgta attgactgat gaatcagtgt ggcgtccagg
      121 acctcctttg tagaggtgta ccgctttctg tctatggtgg tgtcgaagta cttgaaggct
      181 gcaggcgcgc ccaagttggt cagagtaaac aagtggataa tgttttctgc ctgctccctg
      241 atgggcttat ccctgtgctt attgtaagca gaaagcacct tatcgaggtt agcgtcggcg
      301 aggatcactc ttttggagaa ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg
      361 tgttgttcca cgaacagctg cttctgctca ttatcttcgg gagacccttt gagcttttca
      421 tagtggctgg ccagatacaa gaaattaacg tatttagagg gcagtgccag ctcgttacct
      481 ttctgcagct cgcccgcact agcgagcatt cgtttccggc cgttttcaag ctcaaagaga
      541 gagtacttgg gaagcttaat gatgaggtct tttttgacct ctttatatcc tttcgcctcg
      601 agaaagtcga tggggttttt ttcgaagctt gatcgctcca tgattgtgat gcccagcagt
      661 tccttgacgc ttttgagttt tttagacttc cctttctcca ctttggccac aaccagtaca
      721 ctgtaagcga ctgtaggaga atcgaatccg ccgtatttct tggggtccca atcttttttg
      781 cgtgcgatca gcttgtcgct gttccttttc gggaggatac tttccttgga gaagcctccg
      841 gtctgtactt cggtcttttt aacgatgttc acctgcggca tggacaggac cttccggact
      901 gtcgcgaaat ccctaccctt gtcccacacg atttctcctg tttctccgtt tgtttcgata
      961 agtgg
//
