LOCUS       sequence-84876-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  aTc-inducible expression of wild-type Cas9 (S .pyogenes) for 
            bacterial gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pwtCas9-bacteria
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gagatcctta
       61 ctcgagttag tcacctccta gctgactcaa atcaatgcgt gtttcataaa gaccagtgat
      121 ggattgatgg ataagagtgg catctaaaac ttcttttgta gacgtatatc gtttacgatc
      181 aattgttgta tcaaaatatt taaaagcagc gggagctcca agattcgtca acgtaaataa
      241 atgaataata ttttctgctt gttcacgtat tggtttgtct ctatgtttgt tatatgcact
      301 aagaacttta tctaaattgg catctgctaa aataacacgc ttagaaaatt cactgatttg
      361 ctcaataatc tcatctaaat aatgcttatg ctgctccaca aacaattgtt tttgttcgtt
      421 atcttctgga ctacccttca acttttcata atgactagct aaatataaaa aattcacata
      481 tttgcttggc agagccagct catttccttt ttgtaattct ccggcactag ccagcatccg
      541 tttacgaccg ttttctaact caaaaagact atatttaggt agtttaatga ttaagtcttt
      601 tttaacttcc ttatatcctt tagcttctaa aaagtcaatc ggattttttt caaaggaact
      661 tctttccata attgtgatcc ctagtaactc tttaacggat tttaacttct tcgatttccc
      721 tttttccacc ttagcaacca cta
//
