LOCUS       sequence-84877-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  aTc-inducible expression of wild-type Cas9 (S .pyogenes) for 
            bacterial gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pwtCas9-bacteria
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctccacgtt cagctttcgt taaattatca aacttacgtt gagtgattaa cttggcgttt
       61 agaagttgtc tccaatagtt tttcatcttt ttgactactt cttcacttgg aacgttatcc
      121 gatttaccac gatttttatc agaacgcgtt aagaccttat tgtctattga atcgtcttta
      181 aggaaacttt gtggaacaat gtgatcgaca tcataatcac ttaaacgatt aatatctaat
      241 tcttggtcca catacatgtc tcttccattt tggagataat agagatagag cttttcattt
      301 tgcaattgag tattttcaac aggatgctct ttaagaatct gacttcctaa ttctttgata
      361 ccttcttcga ttcgtttcat acgctctcgc gaatttttct ggcccttttg agttgtctga
      421 ttttcacgtg ccatttcaat aacgatattt tctggcttat gccgccccat tactttgacc
      481 aattcatcaa caacttttac agtctgtaaa ataccttttt taatagcagg gctaccagct
      541 aaatttgcaa tatgttcatg taaactatcg ccttgtccag acacttgtgc tttttgaatg
      601 tcttctttaa atgtcaaact atcatcatgg atcagctgca taaaattgcg attggcaaaa
      661 ccatctgatt tcaaaaaatc taatattgtt ttgccagatt gcttatccct aataccatta
      721 atcaattttc gagacaaacg tccccaacca gtataacggc gacgtttaag ctgtttcatc
      781 accttatcat caaagaggtg agcatatgtt ttaagtcttt cctcaatcat ctccctatct
      841 tcaaat
//
