LOCUS       sequence-84878-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Dantas G, Kuhlman B, Callender D, Wong M, Baker D
  TITLE     A large scale test of computational protein design: folding and 
            stability of nine completely redesigned globular proteins.
  JOURNAL   J Mol Biol. 2003 Sep 12. 332(2):449-60.
  PUBMED    12948494
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2003 Sep 12. 332(2):449-60."
COMMENT     Sequence Label: PL1
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             126..148
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..203
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             423..440
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(493..511)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      507..554
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      591..970
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(678..697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     888..909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcga tctcgatccc
       61 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      121 ataattttgt ttaactttaa gaaggagata tacatatgaa agaaaccgct gctgctaaat
      181 tcgaacgcca gcacatggac agcccagatc tgggtacctc aggcgaaaaa actgtggaag
      241 caaactttat cttcgccgat ggtaaaacaa caacaatccg tttcacaggc agtgaggaag
      301 aggctaagaa acgtgtcctg gcgtatgcag aagaattaaa agatacctat ggtgaatact
      361 cagttgatta taaaaacggc ggtgaacaaa ttaacatcaa atttaagggt tcgggcctcg
      421 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      481 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      541 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      601 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      661 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      721 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      781 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
      841 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
      901 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aaggattttg
      961 ccgatttcgg ctattg
//
