LOCUS       sequence-84888-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mCherry-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        48..99
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     50..70
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             134..184
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      200..209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             206..916
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(351..369)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     619..638
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aaaatcaacg ggactttcca aaatgtcgta acaactccgc ccccattgac gcaaatgggc
       61 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
      121 cgctagcgcc accatgggcg tggccgactt gatcaagaag ttcgagtcca tctccaagga
      181 ggagggggat ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat
      241 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      301 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      361 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
      421 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
      481 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      541 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      601 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
      661 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
      721 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
      781 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
      841 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
      901 cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
      961 gaggttttac ttgctttaaa aaaacctccc acacctcccc cctgaacctg aaacataaaa
     1021 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatg
//
