LOCUS       sequence-84893-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mTagBFP-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        29..80
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     31..51
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             115..165
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             187..888
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 aaaatgtcgt aacaactccg cccccattga cgcaaatggg cggtaggcgt gtacggtggg
       61 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc caccatgggc
      121 gtggccgact tgatcaagaa gttcgagtcc atctccaagg aggaggggga tccaccggtc
      181 gccaccatga gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
      241 gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
      301 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
      361 gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac
      421 ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
      481 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
      541 gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc
      601 ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac
      661 gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat
      721 agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga
      781 ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg
      841 gccagatact gcgacctccc tagcaaactg gggcacaagc ttaattaaag cggccgcgac
      901 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaaacctc
      961 ccacacctcc cccctgaacc tgaaacataa aatg
//
