LOCUS       sequence-84894-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: dEos-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..97
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     48..68
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    120..200
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      202..211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             208..891
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MVSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIA
                     RNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
       61 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
      121 ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
      181 cgggcccggg atccaccggt cgccaccatg gtgagtgcga ttaagccaga catgaagatc
      241 aacctccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
      301 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
      361 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa
      421 tatccagacc acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
      481 cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa
      541 ggggacactt tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca
      601 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
      661 ggagtgctga cgggtgatat tcacatggct ttgttgcttg aaggaaatgc ccattaccga
      721 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac
      781 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
      841 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg
      901 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
      961 cccacacctc cccctgaacc tgaacataaa atga
//
