LOCUS       sequence-84896-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 548, Emission: 562.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mOrange-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..78
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     29..49
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    101..181
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      183..192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             189..899
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     602..621
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
       61 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag
      121 atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
      181 tcgccaccat ggtgagcaag ggcgaggaga ataacatggc catcatcaag gagttcatgc
      241 gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      301 gcgagggccg cccctacgag ggctttcaga ccgctaagct gaaggtgacc aagggtggcc
      361 ccctgccctt cgcctgggac atcctgtccc ctcagttcac ctacggctcc aaggcctacg
      421 tgaagcaccc cgccgacatc cccgactact tcaagctgtc cttccccgag ggcttcaagt
      481 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      541 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      601 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
      661 aggacggcgc cctgaagggc gagatcaaga tgaggctgaa gctgaaggac ggcggccact
      721 acacctccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      781 acatcgtcgg catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
      841 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag
      901 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
      961 aaaaacctcc cacacctccc cctgaacctg aaac
//
