LOCUS       sequence-84899-e        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 557, Emission: 585.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mDsRed-C1
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..78
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     29..49
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..797
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     521..540
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    798..863
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    987..1108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1024..1043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1078..1097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
       61 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca
      121 ccatggacaa caccgaggac gtcatcaagg agttcatgca gttcaaggtg cgcatggagg
      181 gctccgtgaa cggccactac ttcgagatcg agggcgaggg cgagggcaag ccctacgagg
      241 gcacccagac cgccaagctg caggtgacca agggcggccc cctgcccttc gcctgggaca
      301 tcctgtcccc ccagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc
      361 ccgactacat gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc atgaacttcg
      421 aggacggcgg cgtggtggag gtgcagcagg actcctccct gcaggacggc accttcatct
      481 acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg cagaagaaga
      541 ctgccggctg ggagccctcc accgagaagc tgtaccccca ggacggcgtg ctgaagggcg
      601 agatctccca cgccctgaag ctgaaggacg gcggccacta cacctgcgac ttcaagaccg
      661 tgtacaaggc caagaagccc gtgcagctgc ccggcaacca ctacgtggac tccaagctgg
      721 acatcaccaa ccacaacgag gactacaccg tggtggagca gtacgagcac gccgaggccc
      781 gccactccgg ctcccagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
      841 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
      901 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
      961 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1081 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg
//
