LOCUS       sequence-84902-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: mAmetrine-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..69
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     20..40
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    92..172
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(180..182,183..185,186..899)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(225..246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
       61 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
      121 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
      181 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      241 gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg cgagggcgat gccaccaacg
      301 gcaagctgac cctgaagttc atctgcacct ccggcaagct gcccgtgccc tggcccaccc
      361 tcgtgaccac cctgtcttac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
      421 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatctcct
      481 tcaaggacga cggcagctac aggacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      541 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      601 agctggagta caacatgaac gtgtgggacg cgtatatcac ggccgacaag cagaagaacg
      661 gcatcaaagc gaacttcaag atcgagcaca acgtcgagga cggcggcgtg cagctcgccg
      721 acgcgtacca gcagaacacc cccatcggcg acggctccgt gctgctgcct gacaaccact
      781 acctgagctt ccagagcaag ctgttcaaag accccaacga gcagcgcgat cacatggtcc
      841 tgctggagtt cgttaccgcc gccgggatca ctcccggcat ggacgagctg tacaagtaag
      901 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
      961 aaaaacctcc cacacctccc cctgaacctg aaac
//
