LOCUS       sequence-84903-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 355, Emission: 424.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa
            K, Nagai T
  TITLE     An ultramarine fluorescent protein with increased photostability and
            pH insensitivity.
  JOURNAL   Nat Methods. 2009 May;6(5):351-3. doi: 10.1038/nmeth.1317. Epub 2009
            Apr 6.
  PUBMED    19349978
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1317"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "351-3"
COMMENT     Sequence Label: Sirius-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..69
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     20..40
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    92..172
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(225..246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(486..505)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             645..896
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKAHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLESVTAAGITLGMDELYK"
ORIGIN
        1 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
       61 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
      121 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
      181 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      241 gcgacgtaaa cggccacagg ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      301 gcaagctgac cctgaagctc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      361 tcgtgaccac cctgcaattc ggcgtgctgt gcttcgcccg ctaccccgac cacatgaagc
      421 agcacgactt cttcaagtcc gccatgcccg atggctacgt ccaggagcgt accatcttct
      481 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      541 tgaaccgcat cgagctgaag ggcttcgact tcaaggagga cggcaacatc ctggggcaca
      601 agctggagta caacgggata agctcaaacg tatatatcac cgccgacaag cagaagaacg
      661 gcatcaaggc ccacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
      721 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
      781 acctgagcgt ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
      841 tgctggagtc cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
      901 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
      961 aaaaacctcc cacacctccc cctgaacctg aaac
//
