LOCUS       sequence-84904-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 
            515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PA-mCherry1-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..69
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     20..40
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    92..172
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             180..890
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(325..343)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
       61 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
      121 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
      181 tggtgagcaa gggcgaggag gataacatgg ccatcattaa ggagttcatg cgcttcaagg
      241 tgcacatgga ggggtccgtg aacggccacg tgttcgagat cgagggcgag ggcgagggcc
      301 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
      361 tcacctggga catcctgagc cctcagttca tgtacggctc caatgcctac gtgaagcacc
      421 ccgccgacat ccccgactac tttaagctgt ccttccccga gggcttcaag tgggagcgcg
      481 tgatgaaatt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      541 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtga
      601 tgcagaagaa gaccatgggc tgggaggccc tctccgagcg gatgtacccc gaggacggcg
      661 ccctgaaggg cgaggtcaag cccagagtga agctgaagga cggcggccac tacgacgctg
      721 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
      781 accgcaagct ggacatcacc agccacaacg aggactacac catcgtggag cagtacgaga
      841 gagccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa gcggccgcga
      901 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
      961 ccacacctcc ccctgaacct gaaacataaa tgaa
//
