LOCUS       sequence-84906-e        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mPlum-Peroxisomes-2
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..141
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     92..112
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    164..244
                     /label=MCS
                     /note="multiple cloning site"
     CDS             252..929
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     653..672
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     847..870
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1068..1148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1105..1124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aagtctccac cccattgacg tcaatgggag tttgtttggc accaaaatca acgggacttt
       61 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      121 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact
      181 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac
      241 cggtggctac catggtgagc aagggcgagg aggtcatcaa ggagttcatg cgcttcaagg
      301 agcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      361 gcccctacga gggcacccag accgccaggc tgaaggtgac caagggtggc cccctgccct
      421 tcgcctggga catcctgtcc cctcagatca tgtacggctc caaggcctac gtgaagcacc
      481 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      541 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      601 gcgagttcat ctacaaggtg aaggtgcgcg gcaccaactt cccctccgac ggccccgtaa
      661 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
      721 ccctgaaggg cgagatgaag atgaggctga ggctgaagga cggcggccac tacgacgccg
      781 aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg
      841 acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
      901 gcgccgaggg ccgccactcc accggcgcct ccggatccaa gctgtagcgg ccgcgactct
      961 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     1021 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1081 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaata aagcattttt
     1141 ttcactgcat tctagttgt
//
