LOCUS       sequence-84907-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-C1
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..141
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     92..112
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             186..863
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     misc_feature    864..929
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1053..1131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagtctccac cccattgacg tcaatgggag tttgtttggc accaaaatca acgggacttt
       61 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      121 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg
      181 ccaccatgag tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa
      241 acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga
      301 gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca
      361 ctgcattcca ttacggcaac agggtattcg ccaaatatcc agacaacata caagactatt
      421 ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg
      481 gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc
      541 gattttatgg taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat
      601 gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattcata
      661 tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag
      721 ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt
      781 taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg
      841 gattgcctga caatgccaga cgatccggac tcagatctcg agctcaagct tcgaattctg
      901 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
      961 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     1021 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     1081 acaataaagc aatagcatca caatttcaca aataagcatt tttttcactg cattct
//
