LOCUS       sequence-84914-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mCherry2-N1
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..68
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     19..39
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    91..171
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      173..182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             179..889
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(324..342)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
       61 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagct
      121 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
      181 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      241 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      301 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
      361 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
      421 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaatt gggagcgcgt
      481 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      541 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
      601 gcagtgtcgt accatgggct gggaggcctc cactgagcgg atgtaccccg aggacggcgc
      661 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
      721 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcga
      781 catcaagttg gacatccttt cccacaacga ggactacacc atcgtggaac agtacgaacg
      841 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcgac
      901 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaa
//
