LOCUS       sequence-84918-e        1147 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 462, Emission: 492.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE
  TITLE     Hue-shifted monomeric variants of Clavularia cyan fluorescent 
            protein: identification of the molecular determinants of color and 
            applications in fluorescence imaging.
  JOURNAL   BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.
  PUBMED    18325109
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2008-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1741-7007-6-13"
COMMENT     Sequence Label: mTFP1-N1
FEATURES             Location/Qualifiers
     source          1..1147
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..111
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     62..82
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    134..214
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      216..225
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             222..932
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     polyA_signal    1054..1134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1091..1110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tttgtttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
       61 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      121 aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc
      181 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg
      241 agaccacaat gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg gagggcaacg
      301 tgaatggcca cgccttcgtg atcgagggcg agggcgaggg caagccctac gacggcacca
      361 acaccatcaa cctggaggtg aaggagggag cccccctgcc cttctcctac gacattctga
      421 ccaccgcgtt cgcctacggc aacagggcct tcaccaagta ccccgacgac atccccaact
      481 acttcaagca gtccttcccc gagggctact cttgggagcg caccatgacc ttcgaggaca
      541 agggcatcgt gaaggtgaag tccgacatct ccatggagga ggactccttc atctacgaga
      601 tacacctcaa gggcgagaac ttccccccca acggccccgt gatgcagaag aagaccaccg
      661 gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag ggcgacgtca
      721 agcacaagct gctgctggag ggcggcggcc accaccgcgt tgacttcaag accatctaca
      781 gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc atcgagatcc
      841 tgaaccacga caaggactac aacaaggtga ccgtttacga gagcgccgtg gcccgcaact
      901 ccaccgacgg catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc
      961 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1021 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1081 tacaaataaa gcaatagcat cacaaatttc acaataaagc atttttttca ctgcattcta
     1141 gttgtgg
//
